Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1
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منابع مشابه
Preparation and purification of glucanase and chitinase from bean leaves.
Glucanase (endo-beta-1, 3-glucan 3-glucanohydrolase, EC 3.2.1.6, laminarinase, callase) and chitinase (poly-beta-1, 4-[2-acetamido-2-deoxy] -d-glucoside glycanohydrolase, EC 3.2.1.14) were extracted from ethylene-treated bean (Phaseolus vulgaris L. cv. Red Kidney) leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns. The glucanase prepared was homogeneous as judged by analyt...
متن کاملPurification and characterization of chitinase from Streptomyces sp. M-20.
Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Streptomyces sp. M-20 and purified by ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and Sephadex G-100 gel filtration. No exochitinase activity was found in the culture filtrate. The molecular mass of the purified chitinase was 20 kDa, estimated by a sodium dodecyl sulfate-polyacrylamide gel electrop...
متن کاملPurification and Properties of Chitinase from Arthrobacter sp. NHB-10.
A chitinase was purified from the culture filtrate of nigeran-degrading Arthrobacter sp. NHB-10 by precipitation with ammonium sulfate and column chromatographies on DEAE-Sephadex A-50 and Superose 12. The final preparation was homogenous in polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was 30,000 and its isoelectric point was 6.8. The optimum pH and temperatur...
متن کاملPurification and Characterization of an Antifungal Chitinase from Arabidopsis thaliana.
Plants exhibit an altered pattern of protein synthesis in response to pathogen invasion and abiotic stress. One of these ;pathogenesis-related' proteins has been identified as chitinase, which is capable of inhibiting fungal growth in vitro. This observation has led to the suggestion that the in vivo role of chitinases is to protect plants against fungal invasion. Here, we report the purificati...
متن کاملPurification and Characterization of Bacterial Chitinase isolated from Crustacean Shells
Four Chitin degrading bacterial strains vibrio aestuarianus, Flavobacterium, Shewenella and Exiguobacterium were isolated from crustacean shells. The strains were confirmed by Biochemical analysis, FAME-GC analysis and 16s rDNA sequencing. The chitinase was purified by a two step chromatographic method and characterized. The enzyme was purified to homogeneity by 6.75 fold with 46% recovery afte...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1996
ISSN: 0378-1097
DOI: 10.1016/0378-1097(96)00036-5